Product Name
Keratin 1 (KRT1), Recombinant Protein
Popular Item
Full Product Name
Recombinant Keratin 1 (KRT1)
Product Gene Name
KRT1 recombinant protein
[Similar Products]
Matching Pairs
Unconjugated
Antibody: Keratin 1 (MBS2028721)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
APC-CY7 Conjugated Antibody: Keratin 1 (KRT1) (MBS2043074)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
PE Conjugated Antibody: Keratin 1 (KRT1) (MBS2043076)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
APC Conjugated Antibody: Keratin 1 (KRT1) (MBS2043078)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
Cy3 Conjugated Antibody: Keratin 1 (KRT1) (MBS2043080)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
FITC Conjugated Antibody: Keratin 1 (KRT1) (MBS2043082)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
HRP Conjugated Antibody: Keratin 1 (KRT1) (MBS2043084)
Immunogen: Keratin 1 (MBS2031731)
Matching Pairs
Biotin Conjugated Antibody: Keratin 1 (KRT1) (MBS2092068)
Immunogen: Keratin 1 (MBS2031731)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2031731 Technical Datasheet
Technical Datasheet PDF
Purity/Purification
> 90%
Concentration
Original Concentration: 200 ug/mL (lot specific)
Organism Species
Rattus norvegicus (Rat)
Source
Prokaryotic expression
Subcellular Location
Membrane, Chromosome
Traits
Freeze-dried powder
Buffer
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Predicted isoelectric point
5.8
Predicted Molecular Mass
13.5kDa
Accurate Molecular Mass
18kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of KRT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for KRT1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
SDS-Page of KRT1 recombinant protein
NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.
NCBI Accession #
AAB47721.1
[Other Products]
UniProt Secondary Accession #
P85925; P86104; Q14720; Q6GSJ0; Q9H298; B2RA01[Other Products]
UniProt Related Accession #
P04264[Other Products]
NCBI Official Full Name
keratin 1
NCBI Official Synonym Full Names
keratin 1, type II
NCBI Official Symbol
KRT1??[Similar Products]
NCBI Official Synonym Symbols
K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK
??[Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 1
UniProt Protein Name
Keratin, type II cytoskeletal 1
UniProt Synonym Protein Names
67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1
UniProt Gene Name
KRT1??[Similar Products]
UniProt Synonym Gene Names
KRTA; CK-1; K1??[Similar Products]
UniProt Entry Name
K2C1_HUMAN
NCBI Summary for KRT1
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Comments for KRT1
K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus
Molecular Function: protein binding; receptor activity; carbohydrate binding; structural molecule activity
Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis
Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis
Research Articles on KRT1
1. In ichthyosis with confetti, a causal de novo KRT1 mutation had a C-terminal frameshift, replacing 22 C-terminal AAs with an alternate 30-AA peptide. It distorted the IF network and mislocalized to the nucleus. Reversion occurred by mitotic recombination.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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