Product Name
Cytokeratin 9 (KRT9), Monoclonal Antibody
Full Product Name
Anti-Cytokeratin 9 Rabbit Monoclonal Antibody
Product Synonym Names
Keratin, type I cytoskeletal 9; Cytokeratin-9; CK-9; Keratin-9; K9; KRT9
Product Gene Name
anti-KRT9 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P35527
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human Cytokeratin 9
Tissue Specificity
Expressed in the terminally differentiated epidermis of palms and soles.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-KRT9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KRT9 antibody
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Product Categories/Family for anti-KRT9 antibody
Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Proteins; cytokeratin
Applications Tested/Suitable for anti-KRT9 antibody
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-KRT9 antibody
WB: 1:1000-1:2000
IHC: 1:50-1:200
IP: 1:50
Western Blot (WB) of anti-KRT9 antibody
Western blot analysis of Cytokeratin 9 expression in HeLa cell lysate (MBS179982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT9
NCBI/Uniprot data below describe general gene information for KRT9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000217.2
[Other Products]
NCBI GenBank Nucleotide #
NP_000217.2
[Other Products]
UniProt Primary Accession #
P35527
[Other Products]
UniProt Secondary Accession #
O00109; Q0IJ47; Q14665[Other Products]
UniProt Related Accession #
P35527[Other Products]
Molecular Weight
62064 MW
NCBI Official Full Name
keratin, type I cytoskeletal 9
NCBI Official Synonym Full Names
keratin 9
NCBI Official Symbol
KRT9??[Similar Products]
NCBI Official Synonym Symbols
K9; CK-9; EPPK
??[Similar Products]
NCBI Protein Information
keratin, type I cytoskeletal 9
UniProt Protein Name
Keratin, type I cytoskeletal 9
UniProt Synonym Protein Names
Cytokeratin-9; CK-9; Keratin-9; K9
UniProt Gene Name
KRT9??[Similar Products]
UniProt Synonym Gene Names
CK-9; K9??[Similar Products]
NCBI Summary for KRT9
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008]
UniProt Comments for KRT9
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Research Articles on KRT9
1. T, as a potential causative mutation in the large Uygur family with epidermolytic palmoplantar keratoderma.">Results identified the missense mutation KRT9 c.487C>T, as a potential causative mutation in the large Uygur family with epidermolytic palmoplantar keratoderma.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
