For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

34betaE12

Mouse

34betaE12 is specific for "high molecular weight" cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in "complex" epithelium. The antibody reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. This antibody reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma.

Affinity Purified

Liquid. In PBS containing 0.1% sodium azide and 1% BSA.

Long term: -20 degree C
Short term: 4 degree C
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.

Small volumes of anti-KRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cell Organelles & Structures

Immunohistochemistry (IHC) (PS), Western Blot (WB)

Immunohistochemistry (paraffin sections; For optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37 degree C for 2 to 4 hours. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection).
General suggested starting dilution is 1:20-1:50.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each appliation.

Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34betaE12), at a dilution of 1:20.

66,039 Da[Similar Products]

keratin 1

keratin, type II cytoskeletal 1

Keratin, type II cytoskeletal 1

KRTA; CK-1; K1??[Similar Products]

K2C1_HUMAN

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoskeleton; extracellular matrix; extracellular space; keratin filament; membrane; nucleus; plasma membrane

Molecular Function: carbohydrate binding; protein binding; receptor activity; structural molecule activity

Biological Process: complement activation, lectin pathway; fibrinolysis; negative regulation of inflammatory response; regulation of angiogenesis; response to oxidative stress; retinal homeostasis

Disease: Epidermolytic Hyperkeratosis; Ichthyosis Hystrix, Curth-macklin Type; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis; Keratosis Palmoplantaris Striata Iii; Palmoplantar Keratoderma, Epidermolytic; Palmoplantar Keratoderma, Nonepidermolytic

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