Full Product Name
ARSA siRNA (Mouse)
Product Synonym Names
AS2; Arylsulfatase A; ASA; Cerebroside-sulfatase
Product Gene Name
ARSA sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P50428
Specificity
ARSA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARSA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ARSA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARSA sirna
siRNA to inhibit ARSA expression using RNA interference
Applications Tested/Suitable for ARSA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ARSA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033843.2
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NCBI GenBank Nucleotide #
NM_009713.4
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UniProt Primary Accession #
P50428
[Other Products]
UniProt Secondary Accession #
Q9DC66[Other Products]
UniProt Related Accession #
P50428[Other Products]
Molecular Weight
53,748 Da
NCBI Official Full Name
arylsulfatase A
NCBI Official Synonym Full Names
arylsulfatase A
NCBI Official Symbol
Arsa??[Similar Products]
NCBI Official Synonym Symbols
ASA; As2; AS-A; As-2; TISP73; AW212749
??[Similar Products]
NCBI Protein Information
arylsulfatase A
UniProt Protein Name
Arylsulfatase A
UniProt Synonym Protein Names
Cerebroside-sulfatase
Protein Family
Arylsulfatase
UniProt Gene Name
Arsa??[Similar Products]
UniProt Synonym Gene Names
As2; ASA??[Similar Products]
UniProt Entry Name
ARSA_MOUSE
UniProt Comments for ARSA
ARSA: Hydrolyzes cerebroside sulfate. Defects in ARSA are a cause of leukodystrophy metachromatic (MLD). MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late- infantile, juvenile and *****. Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD). A clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Arylsulfatase A activity is impaired in multiple sulfatase deficiency due to mutations in SUMF1. SUMF1 mutations result in defective post-translational modification of ARSA at residue Cys- 69 that is not converted to 3-oxoalanine. Belongs to the sulfatase family.
Protein type: Lipid Metabolism - sphingolipid; EC 3.1.6.8; Hydrolase
Cellular Component: extracellular space; extrinsic to external side of plasma membrane; lysosome; cytoplasm; acrosome; plasma membrane; integral to membrane; endosome
Molecular Function: arylsulfatase activity; hydrolase activity; sulfuric ester hydrolase activity; metal ion binding; calcium ion binding; catalytic activity; cerebroside-sulfatase activity
Biological Process: response to ethanol; central nervous system development; binding of sperm to zona pellucida; response to estrogen stimulus; metabolic process; response to methylmercury; autophagy; response to nutrient; response to pH
Research Articles on ARSA
1. Multipotential Neural precursors deficient in arylsulfatase A show a higher ratio of long versus short fatty acid sulfatides, reduction in PDGFRa, decreased AKT phosphorylation, and increased exosomal shedding of PDGFRa.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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