Product Name
ARSA, Recombinant Protein
Full Product Name
ARSA, 21-509aa, Human, His tag, E Coli (Denatured)
Product Synonym Names
Arylsulfatase A; MLD
Product Gene Name
ARSA recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSRPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA
3D Structure
ModBase 3D Structure for P15289
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of ARSA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ARSA recombinant protein
ARSA hydrolyzes cerebrosidesulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. Recombinant human ARSA protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for ARSA recombinant protein
Metabolism
Applications Tested/Suitable for ARSA recombinant protein
SDS-PAGE
SDS-Page of ARSA recombinant protein
NCBI/Uniprot data below describe general gene information for ARSA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000478
[Other Products]
NCBI GenBank Nucleotide #
NM_000487.5
[Other Products]
UniProt Primary Accession #
P15289
[Other Products]
UniProt Secondary Accession #
Q6ICI5; Q96CJ0; B2RCA6; B7XD04; F8WCC8[Other Products]
UniProt Related Accession #
P15289[Other Products]
Molecular Weight
54.3kDa (512aa)
NCBI Official Full Name
arylsulfatase A isoform a
NCBI Official Synonym Full Names
arylsulfatase A
NCBI Official Symbol
ARSA??[Similar Products]
NCBI Official Synonym Symbols
MLD
??[Similar Products]
NCBI Protein Information
arylsulfatase A
UniProt Protein Name
Arylsulfatase A
UniProt Synonym Protein Names
Cerebroside-sulfatase
Protein Family
Arylsulfatase
UniProt Gene Name
ARSA??[Similar Products]
UniProt Synonym Gene Names
ASA??[Similar Products]
NCBI Summary for ARSA
The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]
UniProt Comments for ARSA
Hydrolyzes cerebroside sulfate.
Product References and Citations for ARSA recombinant protein
Matthes,F., et al. (2011) J. Biol. Chem. 286 (20), 17487-17494; Hayashi,T., et al. (2011) Psychiatry Clin. Neurosci. 65 (1), 105-108
Research Articles on ARSA
1. T]+[c.1344dupC]} in the ARSA gene, and both of the variants have been reported as disease-causing mutations previouslyfa">siblings exhibited compound heterozygous variants {[c.302G>T]+[c.1344dupC]} in the ARSA gene, and both of the variants have been reported as disease-causing mutations previouslyfa
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
