For Research Use Only. Not for use in diagnostic procedures.

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS262471 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thymidine Phosphorylase (TP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TP. The ELISA analytical biochemical technique of the MBS262471 kit is based on TP antibody-TP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

50,405 Da

thymidine phosphorylase

thymidine phosphorylase; tdRPase; gliostatin

Thymidine phosphorylase

ECGF1; TP; PD-ECGF??[Similar Products]

TYPH_HUMAN

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Cytokine; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Phosphorylase; EC 2.4.2.4; DNA replication; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)

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