Full Product Name
ESPN siRNA (Mouse)
Product Synonym Names
Espin; Ectoplasmic specialization protein
Product Gene Name
ESPN sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ET47
Specificity
ESPN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ESPN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ESPN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ESPN sirna
siRNA to inhibit ESPN expression using RNA interference
Applications Tested/Suitable for ESPN sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ESPN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_997570.1
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NCBI GenBank Nucleotide #
NM_207687.2
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UniProt Primary Accession #
Q9ET47
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UniProt Secondary Accession #
Q6GYS0; Q6GYS1; Q80ZC0; Q80ZC1; Q80ZC2; A2AK06; A2AK09; A2AK13; B1AWP3; B1AWP6; B1AWQ2[Other Products]
UniProt Related Accession #
Q9ET47[Other Products]
Molecular Weight
28,116 Da
NCBI Official Full Name
espin isoform 1
NCBI Official Synonym Full Names
espin
NCBI Official Symbol
Espn??[Similar Products]
NCBI Official Synonym Symbols
je
??[Similar Products]
NCBI Protein Information
espin
UniProt Protein Name
Espin
UniProt Synonym Protein Names
Ectoplasmic specialization protein
UniProt Gene Name
Espn??[Similar Products]
UniProt Entry Name
ESPN_MOUSE
UniProt Comments for ESPN
ESPN: Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 with or without vestibular involvement (DFNB36). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Actin-binding; Cytoskeletal
Cellular Component: filamentous actin; cell projection; microvillus; cytoskeleton; stereocilium bundle; cytoplasm; stereocilium bundle tip; cell junction; brush border; actin cytoskeleton
Molecular Function: actin filament binding; protein binding; actin binding; SH3 domain binding
Biological Process: positive regulation of filopodium formation; actin filament bundle formation; parallel actin filament bundle formation; sensory perception of sound; actin filament network formation; locomotory behavior; negative regulation of cytoskeleton organization and biogenesis
Research Articles on ESPN
1. We demonstrated for the first time that espin regulates both anchorage-dependent growth and anchorage-independent growth in melanoma cells through G1 arrest and modulates the expression and/or activity of p21Cip1, p27Kip1, Erk, and Akt.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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