Product Name
Thymidine Phosphorylase (TYMP), Polyclonal Antibody
Full Product Name
Anti-Thymidine Phosphorylase Antibody
Product Synonym Names
ECGF1; Thymidine phosphorylase; TP; Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Product Gene Name
anti-TYMP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P19971
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Thymidine Phosphorylase protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
Recombinant full length protein of human Thymidine Phosphorylase
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-TYMP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TYMP antibody
Rabbit polyclonal antibody to Thymidine Phosphorylase
Applications Tested/Suitable for anti-TYMP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-TYMP antibody
Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200
Western Blot (WB) of anti-TYMP antibody
Western blot analysis of Thymidine Phosphorylase expression in A549 (A), THP1 (B) whole cell lysates.
Immunofluorescence (IF) of anti-TYMP antibody
Immunofluorescent analysis of Thymidine Phosphorylase staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
NCBI/Uniprot data below describe general gene information for TYMP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107227.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001113755.2
[Other Products]
UniProt Primary Accession #
P19971
[Other Products]
UniProt Secondary Accession #
Q13390; Q8WVB7; A8MW15; H9KVA0[Other Products]
UniProt Related Accession #
P19971[Other Products]
Molecular Weight
50,405 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP??[Similar Products]
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
??[Similar Products]
NCBI Protein Information
thymidine phosphorylase
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Protein Family
Thymidine phosphorylase
UniProt Gene Name
TYMP??[Similar Products]
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF??[Similar Products]
UniProt Entry Name
TYPH_HUMAN
NCBI Summary for TYMP
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Comments for TYMP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: EC 2.4.2.4; Cytokine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis; Phosphorylase; Transferase; DNA replication
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: phosphorylase activity; platelet-derived growth factor receptor binding; transferase activity, transferring pentosyl groups
Biological Process: DNA replication; mitochondrial genome maintenance; pyrimidine nucleoside catabolic process; pyrimidine nucleoside salvage; pyrimidine nucleotide metabolic process
Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Research Articles on TYMP
1. Gastrointestinal cancer patient carriers of the thymidine phosphorylase rs11479 T allele, with a low platelet count, could be at risk of a poor outcome.
Precautions
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Disclaimer
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