Product Name
Thymidine Phosphorylase (TP), Recombinant Protein
Full Product Name
Recombinant Thymidine Phosphorylase (TP)
Product Gene Name
TP recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- KR DGGHLREADI RNFVHAVIDG RAQDTQIGAM LMAIRLQGMN LEETSVLTRA LAESGQQLEW PKAWHQQLVD KHSTGGVGDK VSLVLAPALA ACGCKVPMIS GRSLGHTGGT LDKLESIPGF GVTQSPEQML HILEEVGCCI VGQSAKLVPA DGILYAARDV TATVDSVPLI TASILSKKAV EGLSTLVVDV KFGGAAVFPD QEKARELAKM LVRVGVSLGL KVAAALTAMD NPLGRSVGHT LEVEEALLCL DGAGPPDLRD LVIRLGGAIL WISGQ
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration
6.4 (lot specific)
Predicted Molecular Mass
32.9kDa
Accurate Molecular Mass (KD)
32kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Mus musculus (Mouse)
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of TP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for TP recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of TP recombinant protein
NCBI/Uniprot data below describe general gene information for TP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001244917.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001257988.1
[Other Products]
UniProt Secondary Accession #
Q13390; Q8WVB7; A8MW15; H9KVA0[Other Products]
UniProt Related Accession #
P19971[Other Products]
Molecular Weight
50,405 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1 proprotein
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP??[Similar Products]
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
??[Similar Products]
NCBI Protein Information
thymidine phosphorylase
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Protein Family
Thymidine phosphorylase
UniProt Gene Name
TYMP??[Similar Products]
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF??[Similar Products]
UniProt Entry Name
TYPH_HUMAN
NCBI Summary for TP
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Comments for TP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Cytokine; DNA replication; Phosphorylase; Motility/polarity/chemotaxis; EC 2.4.2.4; Transferase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding
Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis
Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Research Articles on TP
1. action of thymidine phosphorylase in non-small cell lung cancer: crosstalk with Nrf2 and HO-1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
