For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human PIGW. No significant cross-reactivity or interference between Human PIGW and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PIGW elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282553 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphatidylinositol-glycan biosynthesis class W protein (PIGW) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIGW. The ELISA analytical biochemical technique of the MBS282553 kit is based on PIGW antibody-PIGW antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIGW antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIGW. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIGW in samples. An antibody specific for PIGW has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIGW present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGW is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGW bound in the initial step. The color development is stopped and the intensity of the color is measured.

56,882 Da

phosphatidylinositol glycan anchor biosynthesis class W

phosphatidylinositol-glycan biosynthesis class W protein

Phosphatidylinositol-glycan biosynthesis class W protein

PIG-W??[Similar Products]

The protein encoded by this gene is an inositol acyltransferase that acylates the inositol ring of phosphatidylinositol. This occurs in the endoplasmic reticulum and is a step in the biosynthesis of glycosylphosphatidylinositol (GPI), which anchors many cell surface proteins to the membrane. Defects in this gene are a cause of the age-dependent epileptic encephalopathy West syndrome as well as a syndrome exhibiting hyperphosphatasia and cognitive disability (HPMRS5). [provided by RefSeq, Jul 2017]

PIGW: Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI- anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins. Belongs to the PIGW family.

Protein type: EC 2.3.-.-; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: endoplasmic reticulum membrane; integral component of membrane

Molecular Function: O-acyltransferase activity

Biological Process: GPI anchor metabolic process; preassembly of GPI anchor in ER membrane

Disease: Hyperphosphatasia With Mental Retardation Syndrome 5

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