Anti -MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan, Chondroitin Sulfate Proteoglycan 4 (Melanoma Associated), Chondroitin Sulfate Proteoglycan 4, Chondroitin Sulfate Proteoglycan NG2, CSPG4, MCSPG, MEL-CSPG, Melanoma Chondroitin Sulfate Proteoglyc

For Research Use Only. Not for use in diagnostic procedures.

chromosome: 15; Location: 15q24.2

Monoclonal

IgG1,k

8L721

Mouse

Recognizes human MCSP at ~300kD.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a liquid in Tri-glycine, 150mM sodium chloride, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-CSPG4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. MCSP is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300kD and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and ***** oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumors.

Antibodies; Abs to Proteins

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry.
Dilution: Flow Cytometry: 1:10-1:1000
Western Blot: 1:1000
Immunofluorescence (IC): 1:100
Immunoprecipitation: 1:10-1:100
Immunohistochemistry(Paraffin/frozen): 1:200

250,537 Da[Similar Products]

chondroitin sulfate proteoglycan 4

chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan NG2; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; chondroitin sulfate proteoglycan 4 (melanoma-associated)

Chondroitin sulfate proteoglycan 4

MCSP??[Similar Products]

CSPG4_HUMAN

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq]

NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: lysosomal lumen; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; apical plasma membrane; extracellular region

Molecular Function: signal transducer activity; protein kinase binding

Biological Process: chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; carbohydrate metabolic process; chondroitin sulfate catabolic process; tissue remodeling; pathogenesis; angiogenesis; glial cell migration; dermatan sulfate biosynthetic process; transmembrane receptor protein tyrosine kinase signaling pathway

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