Product Name
Thymidine Phosphorylase (TP), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Thymidine Phosphorylase (TP)
Product Gene Name
anti-TP antibody
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Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
APC-CY7 Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049857)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
PE Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049858)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
APC Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049859)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
Cy3 Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049860)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
FITC Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049861)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
HRP Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2049862)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Thymidine Phosphorylase (MBS2027363)
Biotin Conjugated Antibody: Thymidine Phosphorylase (TP) (MBS2092698)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KR DGGHLREADI RNFVHAVIDG RAQDTQIGAM LMAIRLQGMN LEETSVLTRA LAESGQQLEW PKAWHQQLVD KHSTGGVGDK VSLVLAPALA ACGCKVPMIS GRSLGHTGGT LDKLESIPGF GVTQSPEQML HILEEVGCCI VGQSAKLVPA DGILYAARDV TATVDSVPLI TASILSKKAV EGLSTLVVDV KFGGAAVFPD QEKARELAKM LVRVGVSLGL KVAAALTAMD NPLGRSVGHT LEVEEALLCL
3D Structure
ModBase 3D Structure for Q99N42
Specificity
The antibody is a rabbit polyclonal antibody raised against TP. It has beenselected for its ability to recognize TP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Concentration
200ug/ml (lot specific)
Fragment
Thymidine Phosphorylase (Lys29~Gln305)
Organism Species
Mus musculus (Mouse)
Immunogen
Recombinant Thymidine Phosphorylase (Lys29~Gln305) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049859
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of anti-TP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-TP antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB) of anti-TP antibody
Western Blot: Sample: Recombinant protein.

Immunohistochemistry (IHC) of anti-TP antibody
DAB staining on IHC-P; Samples: Mouse Stomach Tissue)

NCBI/Uniprot data below describe general gene information for TP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_612175.1
[Other Products]
NCBI GenBank Nucleotide #
NM_138302.2
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UniProt Primary Accession #
Q99N42
[Other Products]
UniProt Related Accession #
Q99N42[Other Products]
Molecular Weight
49,336 Da
NCBI Official Full Name
thymidine phosphorylase
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
Tymp??[Similar Products]
NCBI Official Synonym Symbols
TP; Ecgf1; PDECGF; Pdgfec; PD-ECGF; 2900072D10Rik
??[Similar Products]
NCBI Protein Information
thymidine phosphorylase
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
TdRPase
Protein Family
Thymidine phosphorylase
UniProt Gene Name
Tymp??[Similar Products]
UniProt Synonym Gene Names
Ecgf1; TP??[Similar Products]
UniProt Comments for TP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: Cytokine; DNA replication; EC 2.4.2.4; Motility/polarity/chemotaxis; Nucleotide Metabolism - pyrimidine; Phosphorylase; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes
Chromosomal Location of Human Ortholog: 15 E3|15 44.86 cM
Cellular Component: cytosol
Molecular Function: phosphorylase activity; protein homodimerization activity; pyrimidine-nucleoside phosphorylase activity; thymidine phosphorylase activity; transferase activity; transferase activity, transferring glycosyl groups; transferase activity, transferring pentosyl groups
Biological Process: chemotaxis; metabolic process; mitochondrial genome maintenance; organ morphogenesis; pyrimidine base metabolic process; pyrimidine nucleoside metabolic process; regulation of myelination; regulation of transmission of nerve impulse
Research Articles on TP
1. data identify TYMP as an astrocyte-derived permeability factor, and suggest TYMP and VEGFA together promote blood-brain barrier breakdown.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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