Product Name
Thymidine Phosphorylase (TYMP), Recombinant Protein
Full Product Name
Recombinant Human Thymidine Phosphorylase
Product Synonym Names
TYMP Human; Thymidine Phosphorylase Human Recombinant; Thymidine phosphorylase; Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase; TYMP; ECGF1; TP; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
Product Gene Name
TYMP recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGSSHHH HHH SSGLVPRGSH MAPPAP GDFS GEGSQGLPDP SPEPKQLPEL IRMKRDGGRL SEADIRGFVA AVVNGSAQGA QIGAMLMAIR LRGMDLEETS VLTQALAQSG QQLEWPEAWR QQLVDKHSTG GVGDKVSLVL APALAACGCK VPMISGRGLG HTGGTLDKLE SIPGFNVIQS PEQMQVLLDQ AGCCIVGQSE QLVPADGILY AARDVTATVD SLPLITASIL SKKLVEGLSA LVVDVKFGGA AVFPNQEQAR ELAKTLVGVG ASLGLRVAAA LTAMDKPLGR CVGHALEVEE ALLCMDGAGP PDLRDLVTTL GGALLWLSGH AGTQAQGAAR VAAALDDGSA LGRFERMLAA QGVDPGLARA LCSGSPAERR QLLPRAREQE ELLAPADGTV ELVRALPLAL VLHELGAGRS RAGEPLRLGV GAELLVDVGQ RLRRGTPWLR VHRDGPALSG PQSRALQEAL VLSDRAPFAA PSPFAELVLP PQQ
3D Structure
ModBase 3D Structure for P19971
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
The TYMP solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT and 10% glycerol.
Sterile Filtered colorless solution.
Preparation and Storage
TYMP should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Other Notes
Small volumes of TYMP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TYMP recombinant protein
Description: TYMP Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 493 amino acids (11-482 a.a.) and having a molecular mass of 51.3kDa. The TYMP is purified by proprietary chromatographic techniques.
Introduction: Thymidine phosphorylase precursor (TYMP) is a platelet-derived endothelial cell growth factor that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate. TYMP is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. TYMP has a highly restricted target cell specificity acting only on endothelial cells. An increased expression of TYMP is found in a broad array of different solid tumors and inflammatory diseases and is frequently associated with poor prognosis. Mutations in the TYMP gene are linked to mitochondrial neurogastrointestinal encephalomyopathy.
Product Categories/Family for TYMP recombinant protein
ENZYMES; Enzymes; Phosphorylase
NCBI/Uniprot data below describe general gene information for TYMP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001107227.1
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NCBI GenBank Nucleotide #
NM_001113755.2
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UniProt Primary Accession #
P19971
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UniProt Secondary Accession #
Q13390; Q8WVB7; A8MW15; H9KVA0[Other Products]
UniProt Related Accession #
P19971[Other Products]
Molecular Weight
50,405 Da
NCBI Official Full Name
thymidine phosphorylase isoform 1 proprotein
NCBI Official Synonym Full Names
thymidine phosphorylase
NCBI Official Symbol
TYMP??[Similar Products]
NCBI Official Synonym Symbols
TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
??[Similar Products]
NCBI Protein Information
thymidine phosphorylase; gliostatin; tdRPase
UniProt Protein Name
Thymidine phosphorylase
UniProt Synonym Protein Names
Gliostatin; Platelet-derived endothelial cell growth factor; PD-ECGF; TdRPase
Protein Family
Thymidine phosphorylase
UniProt Gene Name
TYMP??[Similar Products]
UniProt Synonym Gene Names
ECGF1; TP; PD-ECGF??[Similar Products]
UniProt Entry Name
TYPH_HUMAN
NCBI Summary for TYMP
This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
UniProt Comments for TYMP
ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Phosphorylase; Nucleotide Metabolism - pyrimidine; Cytokine; EC 2.4.2.4; Motility/polarity/chemotaxis; DNA replication; Transferase
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding
Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; DNA replication; cell differentiation; chemotaxis
Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)
Research Articles on TYMP
1. These results demonstrate that the use of AAV to direct TYMP expression in liver is feasible as a potentially safe gene therapy strategy for Mitochondrial neurogastrointestinal encephalomyopathy.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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