Full Product Name
c-Jun, phosphorylated (Ser63) (jun oncogene, Activator protein 1, AP1, AP-1, c-Jun, p39, Proto-oncogene c-jun, Transcription Factor AP-1, V-jun Avian Sarcoma Virus 17 Oncogene Homolog)
Product Synonym Names
Anti -c-Jun, phosphorylated (Ser63) (jun oncogene, Activator protein 1, AP1, AP-1, c-Jun, p39, Proto-oncogene c-jun, Transcription Factor AP-1, V-jun Avian Sarcoma Virus 17 Oncogene Homolog)
Product Gene Name
anti-C-JUN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_010448.2 (141029845..141031055)
3D Structure
ModBase 3D Structure for P56432
Species Reactivity
Human, Mouse, Rat
Specificity
Recognizes human phosphorylated c-Jun at and around Ser63. Species Crossreactivity: mouse and rat.
Purity/Purification
Supernatant
Supernatant.
Form/Format
Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Immunogen
Synthetic peptide directed against phosphoylated Ser63 of human c-Jun and surrounding amino acids. Epitope: Ser63.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-C-JUN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-C-JUN antibody
Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.
Product Categories/Family for anti-C-JUN antibody
Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-C-JUN antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-C-JUN antibody
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:5000-1:10000
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:100-1:200
Immunoprecipitation: 1:50
NCBI/Uniprot data below describe general gene information for C-JUN. It may not necessarily be applicable to this product.
UniProt Primary Accession #
P56432
[Other Products]
UniProt Related Accession #
P56432[Other Products]
Molecular Weight
35,629 Da[Similar Products]
NCBI Official Full Name
c-Jun
NCBI Official Symbol
C-JUN??[Similar Products]
NCBI Official Synonym Symbols
Jun
??[Similar Products]
NCBI Protein Information
transcription factor AP-1; AP1; activator protein 1; proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog
UniProt Protein Name
Transcription factor AP-1
UniProt Synonym Protein Names
Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog
Protein Family
C-Jun-amino-terminal kinase-interacting protein
UniProt Gene Name
JUN??[Similar Products]
UniProt Entry Name
JUN_PIG
UniProt Comments for C-JUN
Function: Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
Subunit structure: Heterodimer with either FOS or BATF3 or ATF7
By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with MYBBP1A, SP1, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex
By similarity. Interacts with RNF187
Subcellular location: Nucleus
Post-translational modification: Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC
Sequence similarities: Belongs to the bZIP family. Jun subfamily.Contains 1 bZIP domain.
Precautions
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Disclaimer
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