HSPE1/10 kDa heat shock protein; mitochondrial/Hsp10/Early-pregnancy factor/EPF/10 kDa chaperonin/Chaperonin 10/CPN10/Chaperonin 10 Homolog/GroES/GROES/heat shock 10kD protein 1

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of HSPE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761429 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 10 kDa heat shock protein, mitochondrial (HSPE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPE1. The ELISA analytical biochemical technique of the MBS761429 kit is based on HSPE1 antibody-HSPE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HSPE1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HSPE1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HSPE1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HSPE1 can be calculated.

10,932 Da

heat shock protein family E (Hsp10) member 1

10 kDa heat shock protein, mitochondrial

10 kDa heat shock protein, mitochondrial

Hsp10; CPN10; EPF??[Similar Products]

CH10_HUMAN

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; chaperone binding; protein binding; unfolded protein binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein

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