Product Name
BANK1, siRNA
Full Product Name
BANK1 siRNA (Human)
Product Synonym Names
B-cell scaffold protein with ankyrin repeats
Product Gene Name
BANK1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8NDB2
Specificity
BANK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BANK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BANK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BANK1 sirna
siRNA to inhibit BANK1 expression using RNA interference
Applications Tested/Suitable for BANK1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BANK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001077376.2
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NCBI GenBank Nucleotide #
NM_001083907.2
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UniProt Primary Accession #
Q8NDB2
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UniProt Secondary Accession #
Q8N5K8; Q8NB56; Q8WYN5; Q9NWP2; A8K7W8; B0F3S2[Other Products]
UniProt Related Accession #
Q8NDB2[Other Products]
Molecular Weight
73,674 Da
NCBI Official Full Name
B-cell scaffold protein with ankyrin repeats isoform 2
NCBI Official Synonym Full Names
B-cell scaffold protein with ankyrin repeats 1
NCBI Official Symbol
BANK1??[Similar Products]
NCBI Official Synonym Symbols
BANK
??[Similar Products]
NCBI Protein Information
B-cell scaffold protein with ankyrin repeats
UniProt Protein Name
B-cell scaffold protein with ankyrin repeats
Protein Family
B-cell scaffold protein with ankyrin repeats
UniProt Gene Name
BANK1??[Similar Products]
UniProt Entry Name
BANK1_HUMAN
NCBI Summary for BANK1
The protein encoded by this gene is a B-cell-specific scaffold protein that functions in B-cell receptor-induced calcium mobilization from intracellular stores. This protein can also promote Lyn-mediated tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors. Polymorphisms in this gene are associated with susceptibility to systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Comments for BANK1
BANK1: Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2. Genetic variations in BANK1 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q24
Biological Process: negative regulation of translational initiation; negative regulation of protein kinase B signaling cascade; positive regulation of MAPKKK cascade; B cell activation; negative regulation of B cell activation
Disease: Systemic Lupus Erythematosus
Research Articles on BANK1
1. Authors identified a novel balanced reciprocal translocation, t(4;14)(q24;q32.) Sequence analysis identified the partner gene as BANK1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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