14-3-3 protein T-cell; 14-3-3 protein tau; 1433T; 143T; HS1 protein

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total 14-3-3 theta protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1.0 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-14-3-3 theta antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.Nielsen P.J., Biochim. Biophys. Acta 1088:425-428(1991).Leffers H., J. Mol. Biol. 231:982-998(1993).Gevaert K., Nat. Biotechnol. 21:566-569(2003).

Total protein Ab

Western Blot (WB)

Western blotting: 1:500~1:3000

Western blot analysis of extracts from HUVEC cells and HeLa cells, using 14-3-3 theta antibody.

27,764 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta

14-3-3 protein theta

14-3-3 protein theta

1433T_HUMAN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting

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