14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of 14-3-3 theta protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-YWHAQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to 14-3-3 theta

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)

Western blot analysis of 14-3-3 theta expression in HeLa (A), MCF7 (B), SP2/0 (C), rat liver (D) whole cell lysates.

Immunohistochemical analysis of 14-3-3 theta staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of 14-3-3 theta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

27,764 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta

14-3-3 protein theta

14-3-3 protein theta

1433T_HUMAN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting

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