YWHAQ/14-3-3 protein theta/Protein HS1/14-3-3 protein T-cell/14-3-3 protein tau

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of YWHAQ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763827 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 14-3-3 protein theta (YWHAQ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YWHAQ. The ELISA analytical biochemical technique of the MBS763827 kit is based on YWHAQ antibody-YWHAQ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YWHAQ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YWHAQ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human YWHAQ antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human YWHAQ antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human YWHAQ amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human YWHAQ can be calculated.

27,764 Da

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta

14-3-3 protein theta

14-3-3 protein theta

1433T_HUMAN

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; focal adhesion; membrane; mitochondrion; protein complex

Molecular Function: protein binding; protein C-terminus binding; protein domain specific binding; protein N-terminus binding

Biological Process: apoptosis; gene expression; negative regulation of transcription, DNA-dependent; programmed cell death; small GTPase mediated signal transduction; substantia nigra development; transcription initiation from RNA polymerase II promoter

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