For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_000010.10 (45869624..45941567). Location: 10q11.2

Store all reagents at 2-8 degree C

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Small volumes of 5-LO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS101281 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-Lipoxygenase (5-LO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-LO. The ELISA analytical biochemical technique of the MBS101281 kit is based on 5-LO antibody-5-LO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-LO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-LO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

77,983 Da

arachidonate 5-lipoxygenase

arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform

Arachidonate 5-lipoxygenase

LOG5; 5-LO??[Similar Products]

LOX5_HUMAN

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Oxidoreductase; EC 1.13.11.34; Nuclear envelope; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To

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