For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

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Small volumes of 5-LO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2608005 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-lipoxygenase (5-LO) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-LO. The ELISA analytical biochemical technique of the MBS2608005 kit is based on 5-LO antibody-5-LO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-LO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-LO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

56,291 Da

arachidonate 5-lipoxygenase

arachidonate 5-lipoxygenase

Arachidonate 5-lipoxygenase

LOG5; 5-LO; 5-lipoxygenase??[Similar Products]

LOX5_HUMAN

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Lipid Metabolism - arachidonic acid; EC 1.13.11.34; Oxidoreductase; Lipid Metabolism - linoleic acid; Nuclear envelope

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytosol; dendrite; extracellular space; nuclear envelope; nuclear envelope lumen; nuclear matrix; nuclear membrane; sarcolemma

Molecular Function: arachidonate 5-lipoxygenase activity; iron ion binding; protein binding

Biological Process: acute inflammatory response; arachidonic acid metabolic process; leukotriene biosynthetic process; leukotriene metabolic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; positive regulation of vasoconstriction; response to hyperoxia; response to nutrient; sensory perception of pain

Disease: Asthma, Susceptibility To

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