Product Name
5-Lipoxygenase (ALOX5), Recombinant Protein
Popular Item
Full Product Name
5-Lipoxygenase protein
Product Synonym Names
5-Lipoxygenase Antigen; 5 Lipoxygenase Recombinant Protein
Product Gene Name
ALOX5 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P09917
Form/Format
Supplied in 100mM Tris, pH8.0, 5mM EGTA.
Concentration
604 units/ml
22.7mg/ml
Specific Activity: 26.61 Units per mg Protein. (lot specific)
Biohazard
Recombinant protein
Source
Recombinant enzyme isolated from baculovirus over expression system in Sf21 cells
Contaminants
Not determined
Bioactivity
20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 DEG C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equipped
Biological Significance
5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Expression System
Baculovirus
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Upon receipt store at -70°C.
Upon intial thaw, aliquot into single-use volumes and freeze at -70°C.
Shipping: Dry Ice.
Other Notes
Small volumes of ALOX5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for ALOX5 recombinant protein
Proteases, Inhibitors, & Enzymes
Applications Tested/Suitable for ALOX5 recombinant protein
User optimized
NCBI/Uniprot data below describe general gene information for ALOX5. It may not necessarily be applicable to this product.
NCBI Accession #
AAA63212.1
[Other Products]
UniProt Primary Accession #
P09917
[Other Products]
UniProt Secondary Accession #
Q5JQ14; B7ZLS0; E5FPY5; E5FPY7; E5FPY8[Other Products]
UniProt Related Accession #
P09917[Other Products]
Molecular Weight
78,000 kDa[Similar Products]
NCBI Official Full Name
5-lipoxygenase, partial
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
ALOX5??[Similar Products]
NCBI Official Synonym Symbols
5-LO; 5LPG; LOG5; 5-LOX
??[Similar Products]
NCBI Protein Information
arachidonate 5-lipoxygenase
UniProt Protein Name
Arachidonate 5-lipoxygenase
Protein Family
Arachidonate 5-lipoxygenase
UniProt Gene Name
ALOX5??[Similar Products]
UniProt Synonym Gene Names
LOG5; 5-LO; 5-lipoxygenase??[Similar Products]
UniProt Entry Name
LOX5_HUMAN
NCBI Summary for ALOX5
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for ALOX5
5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.
Protein type: EC 1.13.11.34; Oxidoreductase; Nuclear envelope; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol
Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity
Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process
Disease: Asthma, Susceptibility To
Research Articles on ALOX5
1. 5-LOX inhibition reduced apoptotic death, restored the initial IL-2/INF-gamma ratio, and more importantly reverted micro-calpain activation induced by simulated microgravity.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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