Anti-CBLC Antibody (aa444-458) IHC-plus; CBLC; CBL-3; CBL3; RNF57; SH3-binding protein CBL-C; SH3-binding protein CBL-3; CBL-c; CBL-SL; RING finger protein 57; Human CBLC

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Amino acids 444-458 of Human Cbl-c.

Immunoaffinity Purified

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

1.42 mg/ml (lot specific)

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Small volumes of anti-CBLC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CBLC belongs to a family of ubiquitin ligases that are negative regulators of tyrosine kinase signaling downstream of receptor tyrosine kinase and lymphocyte antigen receptors. Monoubiquitin tag promotes sorting of activated receptor and associated proteins into internal vesicles facilitating their lysosomal degradation, while polyubiquitin tag promotes proteasomal degradation. CBLC is expressed in epithelial cells.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

ELISA (1:10000 - 1:50000), IF, IHC-P (5 ug/ml), IP, WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Anti-CBLC antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-Cbl-c Antibody - Immunoprecipitation and Western Blot. Immunoprecipitation and Western blot of Affinity Purified anti-Cbl-c antibody shows detection of a pre-dominant band at ~52 kD corresponding to Cbl-c (arrowhead) in transfected cell lysates (left panel). Lysates are from Hek 293T cells transfected with empty vector or with Cbl-c. The predicted size of Cbl-c is 52 kD. Size markers in kD are shown to the left of the panel. The right panel shows western blotting after first immunoprecipitating with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.

47,418 Da

Cbl proto-oncogene C, E3 ubiquitin protein ligase

E3 ubiquitin-protein ligase CBL-C; RING finger protein 57; SH3-binding protein CBL-3; SH3-binding protein CBL-C; signal transduction protein CBL-C; Cbl proto-oncogene, E3 ubiquitin protein ligase C; Cas-Br-M (murine) ectropic retroviral transforming sequence c; Cas-Br-M (murine) ecotropic retroviral transforming sequence c

E3 ubiquitin-protein ligase CBL-C

CBL3; RNF57??[Similar Products]

CBLC_HUMAN

This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Cbl-c: Regulator of EGFR mediated signal transduction. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleus

Molecular Function: signal transducer activity; zinc ion binding; phosphotyrosine binding; calcium ion binding; SH3 domain binding; ligase activity; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor activity; negative regulation of MAP kinase activity; cell surface receptor linked signal transduction; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination

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