ARF GAP 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein; ARFGAP1; ARF1GAP

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Antigen Affinity Purified

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-ARFGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Recommended dilution: WB:1:500-2000
IHC:1:20-1:200

All lanes: ADP-ribosylation factor GTPase-activating protein 1 antibody at 8ug/ml
Lane 1:PC-3 whole cell lysate
Lane 2:Hela whole cell lysate
Lane 3:mouse brain tissue
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 45,46,32,40 kDa
Observed band size: 45 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using MBS1493690 at dilution 1:100

39,705 Da

ADP ribosylation factor GTPase activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ADP-ribosylation factor GTPase-activating protein 1

ARF1GAP; ARF GAP 1; ARF1 GAP??[Similar Products]

ARFG1_HUMAN

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytosol

Molecular Function: GTPase activator activity; metal ion binding; protein binding

Biological Process: cellular protein metabolic process; COPI coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; positive regulation of GTPase activity; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein transport; regulation of endocytosis; retrograde vesicle-mediated transport, Golgi to ER; unfolded protein response

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