Product Name
FANCL, siRNA
Full Product Name
FANCL siRNA (Mouse)
Product Synonym Names
PHF9; POG; E3 ubiquitin-protein ligase FANCL; Fanconi anemia group L protein homolog; Proliferation of germ cells protein
Product Gene Name
FANCL sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9CR14
Specificity
FANCL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FANCL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FANCL sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FANCL sirna
siRNA to inhibit FANCL expression using RNA interference
Applications Tested/Suitable for FANCL sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FANCL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001264202.1
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NCBI GenBank Nucleotide #
NM_001277273.1
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UniProt Primary Accession #
Q9CR14
[Other Products]
UniProt Secondary Accession #
Q3TGY2; Q9D017[Other Products]
UniProt Related Accession #
Q9CR14[Other Products]
Molecular Weight
41,902 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase FANCL isoform 2
NCBI Official Synonym Full Names
Fanconi anemia, complementation group L
NCBI Official Symbol
Fancl??[Similar Products]
NCBI Official Synonym Symbols
Pog; gcd; Phf9; AW554273; 2010322C19Rik; B230118H11Rik
??[Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase FANCL
UniProt Protein Name
E3 ubiquitin-protein ligase FANCL
UniProt Synonym Protein Names
Fanconi anemia group L protein homolog; Proliferation of germ cells protein
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
Fancl??[Similar Products]
UniProt Synonym Gene Names
Phf9; Pog??[Similar Products]
UniProt Entry Name
FANCL_MOUSE
NCBI Summary for FANCL
This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in ***** males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Comments for FANCL
FANCL: Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth. Defects in FANCL are the cause of Fanconi anemia complementation group L (FANCL). FANCL is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19
Cellular Component: cytoplasm; nuclear envelope; nucleus
Molecular Function: protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity
Biological Process: protein monoubiquitination; gamete generation; protein ubiquitination; DNA repair; response to DNA damage stimulus; regulation of cell proliferation
Research Articles on FANCL
1. The authors report the structure of the human FANCL central domain and show that the RING domain is necessary and sufficient for E2 binding.
Precautions
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Disclaimer
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