Product Name
E3 ubiquitin-protein ligase FANCL (Fancl), Recombinant Protein
Full Product Name
Recombinant Mouse E3 ubiquitin-protein ligase FANCL (Fancl)
Product Gene Name
Fancl recombinant protein
[Similar Products]
Product Synonym Gene Name
Fancl[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
2-375aa, Full Length of Mature Protein
Sequence
DEAEASLLRH FPLLLPQNRE KTVYEGFISA QGSDFHLRIV LPKDLQLKKA RLLCSLQLKN ILNEYHQVVQ QRMKHSPDLM SFMMELKMIL EVALKNKQEL CVQPPSCSFC KDLLTEIGAI GWDKLACVES SFSTIKLKAD DASGRKHLIT VKLKAKYPVE PPDCVVDFPV PFSVSWTPQS SLVDVYSQFL VALETLKVFW DVMDEIDEKT WVLEPEKPPR SATARRIALG KNVSIAIEVD PRHPTMLPEF CFLGADHVTK PLGMKLSGSI HLWDPENSLL QNLKDVLEID FPARSILEES DFSMDCGICY ARHLNGAIPD QVCNNPQCGQ PFHEICLYEW LRGLSTSRQS FNVFFGDCPY CSKPITLKMS GRKP
3D Structure
ModBase 3D Structure for Q9CR14
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Fancl recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Fancl recombinant protein
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group L. Alternative splicing results in two transcript variants encoding different isoforms.
NCBI/Uniprot data below describe general gene information for Fancl. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001264202.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001277273.1
[Other Products]
UniProt Primary Accession #
Q9CR14
[Other Products]
UniProt Secondary Accession #
Q3TGY2; Q9D017[Other Products]
UniProt Related Accession #
Q9CR14[Other Products]
Molecular Weight
41,902 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase FANCL isoform 2
NCBI Official Synonym Full Names
Fanconi anemia, complementation group L
NCBI Official Symbol
Fancl??[Similar Products]
NCBI Official Synonym Symbols
Pog; gcd; Phf9; AW554273; 2010322C19Rik; B230118H11Rik
??[Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase FANCL
UniProt Protein Name
E3 ubiquitin-protein ligase FANCL
UniProt Synonym Protein Names
Fanconi anemia group L protein homolog; Proliferation of germ cells protein; RING-type E3 ubiquitin transferase FANCLCurated
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
Fancl??[Similar Products]
UniProt Synonym Gene Names
Phf9; Pog??[Similar Products]
NCBI Summary for Fancl
This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in ***** males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
UniProt Comments for Fancl
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
Research Articles on Fancl
1. The authors report the structure of the human FANCL central domain and show that the RING domain is necessary and sufficient for E2 binding.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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