Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Lymphocyte antigen 41; Ly-41; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Enpp1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2881656 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (Enpp1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Enpp1. The ELISA analytical biochemical technique of the MBS2881656 kit is based on Enpp1 antibody-Enpp1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Enpp1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Enpp1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

103,076 Da

ectonucleotide pyrophosphatase/phosphodiesterase 1

ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Npps; Pc1; Pdnp1; E-NPP 1; Ly-41; NPPase??[Similar Products]

ENPP1_MOUSE

This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); EC 3.6.1.9; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis; Phosphodiesterase; Membrane protein, integral; EC 3.1.4.1; Carbohydrate Metabolism - starch and sucrose

Cellular Component: cell surface; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; lysosomal membrane; membrane; plasma membrane

Molecular Function: ATP binding; calcium ion binding; catalytic activity; hydrolase activity; insulin receptor binding; metal ion binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; biomineral formation; bone remodeling; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; immune response; inorganic diphosphate transport; metabolic process; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of ossification; negative regulation of protein amino acid autophosphorylation; nucleoside triphosphate catabolic process; phosphate metabolic process; sequestering of triacylglycerol

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