Full Product Name
GLIS3 antibody - N-terminal region
Product Gene Name
anti-GLIS3 antibody
[Similar Products]
Product Synonym Gene Name
ZNF515[Similar Products]
Antibody/Peptide Pairs
GLIS3 peptide (MBS3229886) is used for blocking the activity of GLIS3 antibody (MBS3204920)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: PPASQVSTAC NQISPSLQRA MNAANLNIPP SDTRSLISRE SLASTTLSLT
3D Structure
ModBase 3D Structure for Q8NEA6
Species Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human GLIS3
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-GLIS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GLIS3 antibody
This is a rabbit polyclonal antibody against GLIS3. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: GLIS3 is a member of the GLI-similar zinc finger protein family and has five C2H2-type zinc finger domains. GLIS3 functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH).
Product Categories/Family for anti-GLIS3 antibody
Polyclonal; Transcription Regulation; Developmental Biology; Disease Related; Transcription Factors;
Applications Tested/Suitable for anti-GLIS3 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Immunohistochemistry (IHC) of anti-GLIS3 antibody
Sample Type :
Human Pancreas
Primary Antibody Dilution :
1:10
Secondary Antibody :
Anti-rabbit HRP
Secondary Antibody Dilution :
1:1000
Gene Name :
GLIS3
Submitted by :
Anonymous
Western Blot (WB) of anti-GLIS3 antibody
WB Suggested Anti-GLIS3 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
NCBI/Uniprot data below describe general gene information for GLIS3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_689842
[Other Products]
NCBI GenBank Nucleotide #
NM_152629
[Other Products]
UniProt Primary Accession #
Q8NEA6
[Other Products]
UniProt Related Accession #
Q8NEA6[Other Products]
NCBI Official Full Name
zinc finger protein GLIS3 isoform b
NCBI Official Synonym Full Names
GLIS family zinc finger 3
NCBI Official Symbol
GLIS3??[Similar Products]
NCBI Official Synonym Symbols
NDH; ZNF515
??[Similar Products]
NCBI Protein Information
zinc finger protein GLIS3
UniProt Protein Name
Zinc finger protein GLIS3
UniProt Synonym Protein Names
GLI-similar 3; Zinc finger protein 515
Protein Family
Zinc finger protein
UniProt Gene Name
GLIS3??[Similar Products]
UniProt Synonym Gene Names
ZNF515??[Similar Products]
UniProt Entry Name
GLIS3_HUMAN
NCBI Summary for GLIS3
This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for GLIS3
GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 9p24.2
Cellular Component: nucleoplasm; Golgi apparatus; nucleus
Molecular Function: metal ion binding
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
Disease: Diabetes Mellitus, Neonatal, With Congenital Hypothyroidism
Research Articles on GLIS3
1. We detect a genome-wide significant association at rs10116772 with knee and/or hip total joint replacement; for allele A, an intronic variant in GLIS3, which is expressed in cartilage of osteoarthritis patients.
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