Full Product Name
CPS1 siRNA (Mouse)
Product Synonym Names
Carbamoyl-phosphate synthase [ammonia] mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I
Product Gene Name
CPS1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8C196
Specificity
CPS1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CPS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CPS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CPS1 sirna
siRNA to inhibit CPS1 expression using RNA interference
Applications Tested/Suitable for CPS1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CPS1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001074278.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001080809.2
[Other Products]
UniProt Primary Accession #
Q8C196
[Other Products]
UniProt Secondary Accession #
Q6NX75; A0JNU4[Other Products]
UniProt Related Accession #
Q8C196[Other Products]
Molecular Weight
164,618 Da
NCBI Official Full Name
carbamoyl-phosphate synthase
NCBI Official Synonym Full Names
carbamoyl-phosphate synthetase 1
NCBI Official Symbol
Cps1??[Similar Products]
NCBI Official Synonym Symbols
CPS; D1Ucla3; 4732433M03Rik
??[Similar Products]
NCBI Protein Information
carbamoyl-phosphate synthase [ammonia], mitochondrial
UniProt Protein Name
Carbamoyl-phosphate synthase [ammonia], mitochondrial
UniProt Synonym Protein Names
Carbamoyl-phosphate synthetase I; CPSase I
Protein Family
Carboxypeptidase
UniProt Gene Name
Cps1??[Similar Products]
UniProt Synonym Gene Names
CPSase I??[Similar Products]
UniProt Entry Name
CPSM_MOUSE
NCBI Summary for CPS1
This gene encodes a protein localized to the inner mitochondrial matrix. The encoded protein plays a role in the detoxification of ammonia by catalyzing the first step in the urea cycle in which carbomyl-phosphate is synthesized from ammonia and bicarbonate. Carbamoyl-phosphate is subsequently converted to urea that is excreted by the kidneys. Deficiency of the encoded enzyme leads to an accumulation of ammonia in the blood. High levels of ammonia are toxic to the central nervous system and result in neurological disorders. [provided by RefSeq, Oct 2013]
UniProt Comments for CPS1
CPS1: Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell. Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D). CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation. Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; Mitochondrial; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 6.3.4.16; Ligase
Cellular Component: protein complex; mitochondrion; cytoplasm; mitochondrial inner membrane; nucleus
Molecular Function: carbamoyl-phosphate synthase (ammonia) activity; protein binding; glutamate binding; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; metal ion binding; endopeptidase activity; nucleotide binding; protein complex binding; phospholipid binding; calcium ion binding; catalytic activity; ATP binding; ligase activity
Biological Process: glycogen catabolic process; arginine biosynthetic process; metabolic process; response to lipopolysaccharide; homocysteine metabolic process; positive regulation of vasodilation; proteolysis; nitric oxide metabolic process; nitrogen compound metabolic process; triacylglycerol catabolic process; glutamine catabolic process; urea cycle; anion homeostasis
Research Articles on CPS1
1. The results suggest that elevated isoaspartate and CPS-1, and reduced CA-III levels could serve as biomarkers of hepatocellular injury.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
