H-GLRA1; Glycine Receptor Subunit alpha 1 (GLRA1) Enriched Membrane Preparations; Glycine Receptor Subunit alpha-1 Stable Cell Lines; Glycine Receptor GLRA1 Enriched Membrane Preparations; Glycine receptor 4S kDa subunit; Giycine receptor strychnine-binding subunit

For Research Use Only. Not for use in diagnostic procedures.

The membrane preparations are guaranteed to reach a threshold of 1000 specific DPM/unit.

3.106 cells/mL in freezing medium
Cells: 2 x 1 mL frozen aliquots

Store in liquid nitrogen (vapor phase) immediatly upon receipt.
Stability: The cells were kept in continuous culture for at least 60 days and showed no decrease of receptor expression level.

Small volumes of GLRA1 cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This stable cell line expresses a recombinant human GLRA1. This tetracycline inducible cell line carries 2*Twin-tag at the N-terminus. There is a TEV cleavage site inserted between the tag and the receptor for tag's removal. For all membrane preparations for which radio ligand binding characterization has been conducted, we deliver 1mug/unit, as soon as 1mug is enough to obtain 1000 specific DPM at the Kd concentration of the radio ligand of reference. For all membrane preparations for which radio ligand binding characterization has not yet been conducted, we deliver 10mug/unit.
Tetracycline inducible recombinant human glycine receptor subunit alpha-1 stable CHO cell line.

Human glycine receptor subunit alpha-1 stable CHO-TREx cell line was established using Hygromycin at 150pg/mL. This monoclonal cell line were obtained by limit dilution allowing a homogeneous expression, assesed by flow cytometry (FACS) and confocal immune-imaging.

Receptor Membrane Preparations

51,693 Da[Similar Products]

glycine receptor alpha 1

glycine receptor subunit alpha-1

Glycine receptor subunit alpha-1

The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015]

Glycine receptors are ligand-gated chloride channels (PubMed:23994010, PubMed:25730860). Channel opening is triggered by extracellular glycine (PubMed:2155780, PubMed:7920629, PubMed:14551753, PubMed:16144831, PubMed:22715885, PubMed:22973015, PubMed:25973519, PubMed:9009272). Channel opening is also triggered by taurine and beta-alanine (PubMed:16144831, PubMed:9009272). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization (PubMed:14551753). Plays an important role in the down-regulation of neuronal excitability (PubMed:8298642, PubMed:9009272). Contributes to the generation of inhibitory postsynaptic currents (PubMed:25445488). Channel activity is potentiated by ethanol (PubMed:25973519). Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol ().

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.