Product Name
GLYCINE RECEPTOR ALPHA 1/2 (Glra1), Polyclonal Antibody
Popular Item
Full Product Name
RABBIT ANTI GLYCINE RECEPTOR ALPHA 1/2 (N-TERMINAL)
Product Gene Name
anti-Glra1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse
Form/Format
Purified
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml sterile PBS Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer Solution
Ammonium bicarbonate
Antiserum Preparation
Antisera to rat glycine receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-Glra1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Glra1 antibody
Rabbit anti glycine receptor recognises an epitope within the N-terminal region (NT) of glycine receptor alpha 1 and alpha 2 subunits. Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
Applications Tested/Suitable for anti-Glra1 antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes for anti-Glra1 antibody
Western Blot: This item detects a band of approximately 48 kDa in rat spinal cord lysates.
Immunohistology - Frozen: Maximum Dilution: 1/1000
Western Blotting: Maximum Dilution: 1/1000
NCBI/Uniprot data below describe general gene information for Glra1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037265.1
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NCBI GenBank Nucleotide #
NM_013133.1
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UniProt Secondary Accession #
Q546L6[Other Products]
UniProt Related Accession #
P07727[Other Products]
Molecular Weight
51,686 Da[Similar Products]
NCBI Official Full Name
glycine receptor subunit alpha-1
NCBI Official Synonym Full Names
glycine receptor, alpha 1
NCBI Official Symbol
Glra1??[Similar Products]
NCBI Official Synonym Symbols
GLYRA1
??[Similar Products]
NCBI Protein Information
glycine receptor subunit alpha-1; glycine receptor 48 kDa subunit; glycine receptor strychnine-binding subunit; glycine receptor, alpha 1 subunit
UniProt Protein Name
Glycine receptor subunit alpha-1
UniProt Synonym Protein Names
Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
Protein Family
Glycine receptor
UniProt Gene Name
Glra1??[Similar Products]
UniProt Synonym Gene Names
Glyr??[Similar Products]
UniProt Entry Name
GLRA1_RAT
NCBI Summary for Glra1
glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]
UniProt Comments for Glra1
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:1716350, PubMed:11981023, PubMed:2483325, PubMed:3037383). Channel opening is also triggered by taurine and beta-alanine (PubMed:1716350). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (). Potentiation of channel activity by intoxicating levels ot ethanol contribute to the sedative effects of ethanol ().
Research Articles on Glra1
1. an essential secondary structural requirement for GlyR modulation by ethanol and G protein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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