Full Product Name
CHM siRNA (Rat)
Product Synonym Names
REP1; Rab proteins geranylgeranyltransferase component A 1; Choroideremia protein homolog; Rab escort protein 1; REP-1
Product Gene Name
CHM sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P37727
Specificity
CHM siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CHM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHM sirna
siRNA to inhibit CHM expression using RNA interference
Applications Tested/Suitable for CHM sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_058763.1
[Other Products]
NCBI GenBank Nucleotide #
NM_017067.1
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UniProt Primary Accession #
P37727
[Other Products]
UniProt Related Accession #
P37727[Other Products]
Molecular Weight
72,480 Da
NCBI Official Full Name
rab proteins geranylgeranyltransferase component A 1
NCBI Official Synonym Full Names
choroideremia (Rab escort protein 1)
NCBI Official Symbol
Chm??[Similar Products]
NCBI Protein Information
rab proteins geranylgeranyltransferase component A 1
UniProt Protein Name
Rab proteins geranylgeranyltransferase component A 1
UniProt Synonym Protein Names
Choroideremia protein homolog; Rab escort protein 1; REP-1
Protein Family
Rab proteins geranylgeranyltransferase component
UniProt Gene Name
Chm??[Similar Products]
UniProt Synonym Gene Names
Rep1; REP-1??[Similar Products]
UniProt Entry Name
RAE1_RAT
NCBI Summary for CHM
may function as Rab escort protein (REP); mutations in human homolog associated with choroideremia, a disease of retinal degeneration [RGD, Feb 2006]
UniProt Comments for CHM
CHM: Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation. Defects in CHM are the cause of choroideremia (CHM). An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy. Belongs to the Rab GDI family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase
Cellular Component: Rab-protein geranylgeranyltransferase complex; cytosol
Molecular Function: protein binding; GTPase activator activity; Rab GTPase binding; Rab-protein geranylgeranyltransferase activity
Biological Process: protein geranylgeranylation; blood vessel development; protein targeting to membrane; positive regulation of GTPase activity
Research Articles on CHM
1. RabGGTase has evolved from a GGTase-I-like molecule that 'learned' to interact with a recycling factor (GDI) that, in turn, eventually gave rise to REP1.
Precautions
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Disclaimer
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