Full Product Name
EHD2 Antibody Affinity Purified
Product Synonym Names
EH domain containing 2; EH domain containing 3; EHD 2; EHD3; FLJ96617; PAST 2 antibody
Product Gene Name
anti-EHD2 antibody
[Similar Products]
Matching Pairs
Antibody: EHD2 (MBS540294)
Positive Control: EHD2 (MBS542726)
Antibody/Peptide Pairs
EHD2 peptide (MBS543619) is used for blocking the activity of EHD2 antibody (MBS540294)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NZN4
Species Reactivity
Human, Mouse, Rat
Form/Format
Affinity Purified
Concentration
0.55 ug/ul in antibody stabilization buffer (lot specific)
Immunogen
Synthetic peptide taken within amino acid 420-450 on human EH-Domain Containing Protein 2.
Expression
Highly expressed in heart and moderately expressed in placenta, lung, and skeletal muscle.
Molecular Function
ATP binding;GTPase activity;nucleic acid binding;calcium ion binding;GTP binding
Structure
Homodimer and homooligomer. Interacts with EHD1 and MYOF. May also interact with EHD3 and EHD4.
Subcellular Location
Cell membrane, Endosome, Membrane
Preparation and Storage
-20? degree C for long term storage
Other Notes
Small volumes of anti-EHD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EHD2 antibody
Affinity Purified EH domain-containing protein 2 Antibody
Plays a role in membrane reorganization in response to nucleotide hydrolysis
Product Categories/Family for anti-EHD2 antibody
Primary Antibodies; Epigenetics; DNA / RNA; EHD2 Antibody
Applications Tested/Suitable for anti-EHD2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-EHD2 antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Western Blot: 1:500
NCBI/Uniprot data below describe general gene information for EHD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055416.2
[Other Products]
NCBI GenBank Nucleotide #
NM_014601.3
[Other Products]
UniProt Primary Accession #
Q9NZN4
[Other Products]
UniProt Secondary Accession #
Q96CB6; B2RDH9; B4DNU6[Other Products]
UniProt Related Accession #
Q9NZN4[Other Products]
Molecular Weight
46,059 Da
NCBI Official Full Name
EH domain-containing protein 2
NCBI Official Synonym Full Names
EH domain containing 2
NCBI Official Symbol
EHD2??[Similar Products]
NCBI Official Synonym Symbols
PAST2
??[Similar Products]
NCBI Protein Information
EH domain-containing protein 2
UniProt Protein Name
EH domain-containing protein 2
UniProt Synonym Protein Names
PAST homolog 2Curated
Protein Family
EH domain-containing protein
UniProt Gene Name
EHD2??[Similar Products]
UniProt Entry Name
EHD2_HUMAN
NCBI Summary for EHD2
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
UniProt Comments for EHD2
EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane
Molecular Function: ATP binding; calcium ion binding; GTP binding; hydrolase activity; nucleic acid binding; protein binding; protein domain specific binding
Biological Process: blood coagulation; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endocytosis; metabolic process
Research Articles on EHD2
1. Data suggest that the EH-domain containing 2 protein (EHD2) NPF phenylalanine residue is crucial for EHD2 localization to the plasma membrane, whereas the proline residue is essential for EHD2 dimerization and binding.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
