Anti -ARFGAP1 (ADP Ribosylation Factor GTPase Activating Protein 1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 20; NC_000020.10 (61904165..61921142). Location: 20q13.33

Polyclonal

IgG

Goat

Recognizes ARFGAP1 (ADP-ribosylation factor GTPase activating protein 1).

Affinity Purified
Purified IgG was prepared from whole serum by affinity chromatography.

Purified IgG - liquid in TRIS buffered saline pH 7.3 with 0.02% sodium azide, 0.5% BSA.

-20 degree C

Small volumes of anti-ARFGAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This protein is thought to promote hydrolysis of ARF1 bound GTP, being required for the dissociation of coat proteins from Golgi derived membranes and vesicles. ARFGAP1 is widely expressed, being found at high levels in endocrine glands.

Antibodies; Abs to GTPase, Rab, Ras, Rho Proteins

Western Blot (WB)

Western Blotting: 1-3ug/ml, Optimal working dilutions to be determined by researcher.

44,669 Da[Similar Products]

ADP-ribosylation factor GTPase activating protein 1

ADP-ribosylation factor GTPase-activating protein 1; ARF1 GAP; ARF GAP 1; GAP protein; OTTHUMP00000031551; OTTHUMP00000196668; OTTHUMP00000196669; OTTHUMP00000226307; ARF1-directed GTPase-activating protein; ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor 1 GTPase-activating protein

ADP-ribosylation factor GTPase activating protein 1 isoform a variant

Q53F62_HUMAN

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, ARF; GAPs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: synapse; cytosol

Molecular Function: protein binding; zinc ion binding; GTPase activator activity

Biological Process: protein transport; COPI coating of Golgi vesicle; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; retrograde vesicle-mediated transport, Golgi to ER; positive regulation of GTPase activity

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