Product Name
KMT2D, siRNA
Full Product Name
KMT2D siRNA (Human)
Product Synonym Names
ALR; MLL2; MLL4; Histone-lysine N-methyltransferase 2D; Lysine N-methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2
Product Gene Name
KMT2D sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O14686
Specificity
KMT2D siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KMT2D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KMT2D sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KMT2D sirna
siRNA to inhibit KMT2D expression using RNA interference
Applications Tested/Suitable for KMT2D sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KMT2D. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003473.3
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NCBI GenBank Nucleotide #
NM_003482.3
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UniProt Primary Accession #
O14686
[Other Products]
UniProt Secondary Accession #
O14687[Other Products]
UniProt Related Accession #
O14686[Other Products]
Molecular Weight
593,677 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2D
NCBI Official Synonym Full Names
lysine (K)-specific methyltransferase 2D
NCBI Official Symbol
KMT2D??[Similar Products]
NCBI Official Synonym Symbols
ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114
??[Similar Products]
NCBI Protein Information
histone-lysine N-methyltransferase 2D
UniProt Protein Name
Histone-lysine N-methyltransferase 2D
UniProt Synonym Protein Names
ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
KMT2D??[Similar Products]
UniProt Synonym Gene Names
ALR; MLL2; MLL4; Lysine N-methyltransferase 2D??[Similar Products]
UniProt Entry Name
KMT2D_HUMAN
NCBI Summary for KMT2D
The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]
UniProt Comments for KMT2D
MLL4: Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. Defects in MLL2 are the cause of Kabuki syndrome type 1 (KABUK1). A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; EC 2.1.1.43; Transcription regulation; Methyltransferase, protein lysine; Methyltransferase
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific)
Biological Process: oogenesis; oocyte growth; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to estrogen stimulus; histone H3-K4 methylation; positive regulation of estrogen receptor signaling pathway; chromatin silencing; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter
Disease: Kabuki Syndrome 1
Research Articles on KMT2D
1. MLL4 (KMT2D) is a major H3K4 mono- and di-methyltransferase with partial functional redundancy with MLL3 (KMT2C) in mouse and human cells. MLL4 is enriched on enhancers and is required for enhancer activation, cell-type-specific gene expression and cell differentiation.
Precautions
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Disclaimer
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