Full Product Name
Goat anti-EHD2 Antibody
Product Synonym Names
EHD2; PAST2; EH-domain containing 2; EH domain containing 2; FLJ96617; EHD2 antibody; PAST2 antibody; EH-domain containing 2 antibody; EH domain containing 2 antibody; FLJ96617 antibody
Product Gene Name
anti-EHD2 antibody
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Antibody/Peptide Pairs
EHD2 peptide (MBS426788) is used for blocking the activity of EHD2 antibody (MBS420993)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
CRLVPPSKRR HKGSA
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.
Note
This antibody is expected to recognise EHD1 protein as well as EHD2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-EHD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-EHD2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-EHD2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB) of anti-EHD2 antibody
(0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.
Western Blot (WB) of anti-EHD2 antibody
HEK293 overexpressing EHD2 (RC204848) and probed (mock transfection in first lane), tested by Origene.
NCBI/Uniprot data below describe general gene information for EHD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055416.2
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NCBI GenBank Nucleotide #
NM_014601.3
[Other Products]
UniProt Secondary Accession #
Q96CB6; B2RDH9; B4DNU6[Other Products]
UniProt Related Accession #
Q9NZN4[Other Products]
Molecular Weight
46,059 Da
NCBI Official Full Name
EH domain-containing protein 2
NCBI Official Synonym Full Names
EH domain containing 2
NCBI Official Symbol
EHD2??[Similar Products]
NCBI Official Synonym Symbols
PAST2
??[Similar Products]
NCBI Protein Information
EH domain-containing protein 2
UniProt Protein Name
EH domain-containing protein 2
UniProt Synonym Protein Names
PAST homolog 2Curated
Protein Family
EH domain-containing protein
UniProt Gene Name
EHD2??[Similar Products]
UniProt Entry Name
EHD2_HUMAN
NCBI Summary for EHD2
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
UniProt Comments for EHD2
EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane
Molecular Function: ATP binding; calcium ion binding; GTP binding; hydrolase activity; nucleic acid binding; protein binding; protein domain specific binding
Biological Process: blood coagulation; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endocytosis; metabolic process
Research Articles on EHD2
1. Data suggest that the EH-domain containing 2 protein (EHD2) NPF phenylalanine residue is crucial for EHD2 localization to the plasma membrane, whereas the proline residue is essential for EHD2 dimerization and binding.
Precautions
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