Full Product Name
EHD2 siRNA (Mouse)
Product Synonym Names
EH domain-containing protein 2
Product Gene Name
EHD2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8BH64
Specificity
EHD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EHD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EHD2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EHD2 sirna
siRNA to inhibit EHD2 expression using RNA interference
Applications Tested/Suitable for EHD2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EHD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_694708.2
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NCBI GenBank Nucleotide #
NM_153068.3
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UniProt Primary Accession #
Q8BH64
[Other Products]
UniProt Secondary Accession #
Q8BL28[Other Products]
UniProt Related Accession #
Q8BH64[Other Products]
Molecular Weight
61,174 Da
NCBI Official Full Name
EH domain-containing protein 2
NCBI Official Synonym Full Names
EH-domain containing 2
NCBI Official Symbol
Ehd2??[Similar Products]
NCBI Official Synonym Symbols
BC027084; C130052H20Rik
??[Similar Products]
NCBI Protein Information
EH domain-containing protein 2
UniProt Protein Name
EH domain-containing protein 2
Protein Family
EH domain-containing protein
UniProt Gene Name
Ehd2??[Similar Products]
UniProt Entry Name
EHD2_MOUSE
UniProt Comments for EHD2
EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
Protein type: Vesicle
Cellular Component: membrane; recycling endosome membrane; perinuclear region of cytoplasm; plasma membrane; caveola; endosome
Molecular Function: protein domain specific binding; protein binding; GTP binding; hydrolase activity; metal ion binding; nucleotide binding; calcium ion binding; ATP binding
Biological Process: endocytosis; cortical actin cytoskeleton organization and biogenesis; actin cytoskeleton organization and biogenesis; endocytic recycling
Research Articles on EHD2
1. The helical domain of EHD2 is inserted into the membrane.The N terminus domain regulates caveolar targeting of EHD2.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
