Full Product Name
EHD2 siRNA (Human)
Product Synonym Names
PAST2; EH domain-containing protein 2; PAST homolog 2
Product Gene Name
EHD2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9NZN4
Specificity
EHD2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EHD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EHD2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EHD2 sirna
siRNA to inhibit EHD2 expression using RNA interference
Applications Tested/Suitable for EHD2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EHD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055416.2
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NCBI GenBank Nucleotide #
NM_014601.3
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UniProt Primary Accession #
Q9NZN4
[Other Products]
UniProt Secondary Accession #
Q96CB6; B2RDH9; B4DNU6[Other Products]
UniProt Related Accession #
Q9NZN4[Other Products]
Molecular Weight
46,059 Da
NCBI Official Full Name
EH domain-containing protein 2
NCBI Official Synonym Full Names
EH-domain containing 2
NCBI Official Symbol
EHD2??[Similar Products]
NCBI Official Synonym Symbols
PAST2
??[Similar Products]
NCBI Protein Information
EH domain-containing protein 2
UniProt Protein Name
EH domain-containing protein 2
UniProt Synonym Protein Names
PAST homolog 2
Protein Family
EH domain-containing protein
UniProt Gene Name
EHD2??[Similar Products]
UniProt Synonym Gene Names
PAST2??[Similar Products]
UniProt Entry Name
EHD2_HUMAN
NCBI Summary for EHD2
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
UniProt Comments for EHD2
EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: recycling endosome membrane; perinuclear region of cytoplasm; plasma membrane; endosome membrane; caveola; nucleus
Molecular Function: protein domain specific binding; protein binding; GTP binding; nucleic acid binding; hydrolase activity; calcium ion binding; ATP binding
Biological Process: metabolic process; endocytosis; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; blood coagulation
Research Articles on EHD2
1. Downregulation of EHD2 was associated with migration and invasion by abrogating the expression of Rac1 in breast cancer patients.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
