EH domain-containing protein 2; PAST homolog 2; EHD2; PAST2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This EHD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 415-449 amino acids from the C-terminal region of human EHD2.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-EHD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).

Crown Antibodies

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

IHC~~1:25
WB~~1:1000

Immunohistochemical analysis of paraffin-embedded H. heart section using EHD2 Antibody (C-term). MBS9213953 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. placenta section using EHD2 Antibody (C-term). MBS9213953 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from A549, Hela cell line, human placenta, mouse lung, rat lung tissue (from left to right), using EHD2 Antibody (C-term). MBS9213953 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

61161

EH-domain containing 2

EH domain-containing protein 2

EH domain-containing protein 2

PAST2??[Similar Products]

EHD2_HUMAN

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: recycling endosome membrane; perinuclear region of cytoplasm; plasma membrane; endosome membrane; caveola; nucleus

Molecular Function: protein domain specific binding; protein binding; GTP binding; nucleic acid binding; hydrolase activity; calcium ion binding; ATP binding

Biological Process: metabolic process; endocytosis; cortical actin cytoskeleton organization and biogenesis; blood coagulation; endocytic recycling

Pohl U.,et al.Genomics 63:255-262(2000).
Benjamin S.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Aboulaich N.,et al.Biochem. J. 383:237-248(2004).

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