Product Name
GLRA1, siRNA
Full Product Name
GLRA1 siRNA (Rat)
Product Synonym Names
GLYR; Glycine receptor subunit alpha-1; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
Product Gene Name
GLRA1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07727
Specificity
GLRA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GLRA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of GLRA1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GLRA1 sirna
siRNA to inhibit GLRA1 expression using RNA interference
Applications Tested/Suitable for GLRA1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for GLRA1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037265.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013133.1
[Other Products]
UniProt Primary Accession #
P07727
[Other Products]
UniProt Secondary Accession #
Q546L6[Other Products]
UniProt Related Accession #
P07727[Other Products]
Molecular Weight
51,686 Da
NCBI Official Full Name
glycine receptor subunit alpha-1
NCBI Official Synonym Full Names
glycine receptor, alpha 1
NCBI Official Symbol
Glra1??[Similar Products]
NCBI Official Synonym Symbols
GLYRA1
??[Similar Products]
NCBI Protein Information
glycine receptor subunit alpha-1
UniProt Protein Name
Glycine receptor subunit alpha-1
UniProt Synonym Protein Names
Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
Protein Family
Glycine receptor
UniProt Gene Name
Glra1??[Similar Products]
UniProt Synonym Gene Names
Glyr??[Similar Products]
UniProt Entry Name
GLRA1_RAT
NCBI Summary for GLRA1
glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]
UniProt Comments for GLRA1
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:1716350, PubMed:11981023, PubMed:2483325, PubMed:3037383). Channel opening is also triggered by taurine and beta-alanine (PubMed:1716350). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (). Potentiation of channel activity by intoxicating levels ot ethanol contribute to the sedative effects of ethanol ().
Research Articles on GLRA1
1. an essential secondary structural requirement for GlyR modulation by ethanol and G protein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
