Anti -Uromucoid

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 16; NC_000016.9 (20344373..20364037, complement). Location: 16p12.3

Polyclonal

IgG

Sheep

Gives a single arc when tested by IEP against fresh urine. No arcs are visible when tested by IEP against serum. Identity has been confirmed by double diffusion (Ouchterlony) against uromucoid and a known anti-uromucoid. Shown to be specfic by gel diffusion techniques.

Purified
~95%. Adsorbed to monospecificity by use of solid-phase adsorbants. The titer is adjusted so that inter-batch variation is within 10%. Product is 0.2um filtered.

Supplied as a liquid glycine buffered saline pH 7.4 0.1% sodium azide, 0.1% e-amino-n-caproic acid, 0.01% benzamidine, 1mM?EDTA.

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-UMOD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Proteins

Gel Shift Assay (GS/EMSA)

Suitable for use in RID, Double Diffusion and IEP.
Dilution: RID: 10ul antiserum/cm2 in gel vs 5ul uromucoid 50mg/L (Neat 1:5).
Double Diffusion: 10ul antiserum vs 3ul uromucoid 50mg/L.
IEP: 100ul antiserum vs 5ul uromucoid 50mg/L. The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.

69,761 Da[Similar Products]

uromodulin

uromodulin; uromucoid; OTTHUMP00000162212; OTTHUMP00000162213; Tamm-Horsfall glycoprotein; tamm-Horsfall urinary glycoprotein; uromodulin (uromucoid, Tamm-Horsfall glycoprotein)

Uromodulin

UROM_HUMAN

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

UMOD: Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial. Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1). HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis. Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2). MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by ***** onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade. Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI). GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: spindle pole; Golgi apparatus; extracellular space; extrinsic to membrane; basolateral plasma membrane; apical plasma membrane; cytoplasmic vesicle; lipid raft

Molecular Function: IgG binding; calcium ion binding

Biological Process: response to organic substance; heterophilic cell adhesion; negative regulation of cell proliferation; leukocyte adhesion; cellular defense response; excretion; chemical homeostasis

Disease: Hyperuricemic Nephropathy, Familial Juvenile, 1; Glomerulocystic Kidney Disease With Hyperuricemia And Isosthenuria; Medullary Cystic Kidney Disease 2

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