EYA4; eyes absent homolog 4 (Drosophila); HGNC: 3522; DFNA10; OTTHUMP00000040267; dJ78N10.1 (eyes absent (Drosophila) homolog 4); deafness, autosomal dominant 10; eyes absent 4; RP11-704J17.4; CMD1J; DFNA10; EYA4 antibody; eyes absent homolog 4 (Drosophila) antibody; HGNC: 3522 antibody; DFNA10 antibody; OTTHUMP00000040267 antibody; dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody; deafness; autosomal dominant 10 antibody; eyes absent 4 antibody; RP11-704J17.4 antibody; CMD1J antibody; DFNA10 antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-EYA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA)

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide.

63,911 Da

EYA transcriptional coactivator and phosphatase 4

eyes absent homolog 4

Eyes absent homolog 4

EYA4_HUMAN

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

EYA4: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye. Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10). DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the HAD-like hydrolase superfamily. EYA family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; nucleus

Molecular Function: metal ion binding; protein binding; protein tyrosine phosphatase activity

Biological Process: anatomical structure morphogenesis; chromatin modification; DNA repair; multicellular organismal development; regulation of transcription, DNA-dependent; sensory perception of sound; transcription, DNA-dependent; visual perception

Disease: Cardiomyopathy, Dilated, 1j; Deafness, Autosomal Dominant 10

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