Lrp2/gp330/Megalin/LRP-2/Glycoprotein 330

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of Lrp2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS764144 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Low-density lipoprotein receptor-related protein 2 (Lrp2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lrp2. The ELISA analytical biochemical technique of the MBS764144 kit is based on Lrp2 antibody-Lrp2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lrp2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lrp2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Lrp2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Lrp2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Lrp2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Lrp2 can be calculated.

519,208 Da

low density lipoprotein receptor-related protein 2

low-density lipoprotein receptor-related protein 2

Low-density lipoprotein receptor-related protein 2

LRP-2; gp330??[Similar Products]

LRP2_MOUSE

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Cellular Component: apical part of cell; apical plasma membrane; brush border; brush border membrane; coated pit; cytoplasm; endocytic vesicle; endoplasmic reticulum; endosome; extracellular space; Golgi apparatus; integral to membrane; lipid raft; lysosomal membrane; membrane; protein complex; receptor complex

Molecular Function: calcium ion binding; hemoglobin binding; hormone binding; lipoprotein transporter activity; low-density lipoprotein receptor binding; metal ion binding; PDZ domain binding; protein binding; protein complex binding; receptor activity; SH3 domain binding

Biological Process: cell proliferation; endocytosis; endosome transport; forebrain development; heart development; hemoglobin import; hormone secretion; lipoprotein transport; receptor-mediated endocytosis; response to X-ray; transcytosis; vitamin metabolic process

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