Product Name
BD3 (Defb3), Recombinant Protein
Full Product Name
BD3, Recombinant, Human (beta Defensin-3)
Product Gene Name
Defb3 recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Chromosome Location
Chromosome: 8; NC_000074.5 (19293361..19295339). Location: 8; 8
3D Structure
ModBase 3D Structure for Q9WTL0
Purity/Purification
Highly Purified
95% as determined by RP-HPLC and SDS-PAGE. Purified by proprietary chromatographic techniques.
Form/Format
Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH20 to >0.1mg/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of Defb3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Defb3 recombinant protein
Recombinant Human BD-3 produced in E.coli is a single, non-glycosylated polypeptide chain containing 45 amino acids and having a molecular mass of 5.16kD.
BBeta-Defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta-Defensins which are distinguished from alpha-Defensins by the connectivity pattern of their three intramolecular disulfide bonds. The 45 aa mature human BD3 shares 38% and 33% aa sequence identity with mouse and rat BD3, respectively. It shares 18%-36% aa sequence identity with other human beta-Defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is upregulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds. BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression. In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S. BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast. BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability. Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties. BD3 inhibits viral infectivity by interacting directly with HIV-1 plus its coreceptor CXCR4, and with HSV glycoprotein B plus its receptor heparan sulfate, and by forming a protective coating on the surface of influenza virus target cells.
Product Categories/Family for Defb3 recombinant protein
Growth Factors, Cytokines; Growth Factors-Defensin
NCBI/Uniprot data below describe general gene information for Defb3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038784.1
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NCBI GenBank Nucleotide #
NM_013756.2
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UniProt Primary Accession #
Q9WTL0
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UniProt Secondary Accession #
Q29R78[Other Products]
UniProt Related Accession #
Q9WTL0[Other Products]
Molecular Weight
5.16kD[Similar Products]
NCBI Official Full Name
beta-defensin 3
NCBI Official Synonym Full Names
defensin beta 3
NCBI Official Symbol
Defb3??[Similar Products]
NCBI Official Synonym Symbols
BD-3; MGC129397
??[Similar Products]
NCBI Protein Information
beta-defensin 3; OTTMUSP00000022575
UniProt Protein Name
Beta-defensin 3
UniProt Synonym Protein Names
Defensin, beta 3
Protein Family
Beta-defensin
UniProt Gene Name
Defb3??[Similar Products]
UniProt Synonym Gene Names
Bd3??[Similar Products]
UniProt Entry Name
DEFB3_MOUSE
UniProt Comments for Defb3
Function: Antimicrobial activity against Gram-negative bacteria E.coli and P.aeruginosa. Ref.1
Subcellular location: Secreted.
Tissue specificity: Highest expression in salivary glands, epididymis, ovary and pancreas and to a lesser extent in lung, liver and brain. Low or no expression in skeletal muscle and tongue. Ref.1 Ref.3
Induction: By bacterial infection. Ref.1
Sequence similarities: Belongs to the beta-defensin family. LAP/TAP subfamily.
Research Articles on Defb3
1. Mouse beta-defensin 3 was strongly up-regulated in colonic epithelium of 15 (but not 5) week-old IL-2(-/-) mice and in dextran-sulfate-induced chronic (but not acute) colitis, but not in IL-2(+/-) 15-week-old mice.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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