Product Name
BD3 (Defb3), Polyclonal Antibody
Full Product Name
BD3 (beta-Defensin 3)
Product Synonym Names
Anti -BD3 (beta-Defensin 3)
Product Gene Name
anti-Defb3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 8; NC_000074.5 (19293361..19295339). Location: 8; 8
3D Structure
ModBase 3D Structure for Q9WTL0
Species Reactivity
Human, Mouse
Specificity
Recognizes both mouse and human defensin-3, but not other beta-defensins.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Immunogen
A 13aa peptide mapping at the N-terminus of mature mouse defensin-3 and a 15aa peptide mapping at the N-terminus of mature human defensin-3. The two peptides were mixed and coupled to KLH prior to immunization.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-Defb3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Defb3 antibody
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by conserved tri-disulfides and a largely b-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human a- and b-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a- and b- based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified to date. Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologs of the human beta-defensin have also been identified.
Product Categories/Family for anti-Defb3 antibody
Antibodies; Abs to Defensin
Applications Tested/Suitable for anti-Defb3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Defb3 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL technique.
Optimal dilution determined by the researcher.
NCBI/Uniprot data below describe general gene information for Defb3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038784.1
[Other Products]
NCBI GenBank Nucleotide #
NM_013756.2
[Other Products]
UniProt Primary Accession #
Q9WTL0
[Other Products]
UniProt Secondary Accession #
Q29R78[Other Products]
UniProt Related Accession #
Q9WTL0[Other Products]
Molecular Weight
7,126 Da[Similar Products]
NCBI Official Full Name
beta-defensin 3
NCBI Official Synonym Full Names
defensin beta 3
NCBI Official Symbol
Defb3??[Similar Products]
NCBI Official Synonym Symbols
BD-3; MGC129397
??[Similar Products]
NCBI Protein Information
beta-defensin 3; OTTMUSP00000022575
UniProt Protein Name
Beta-defensin 3
UniProt Synonym Protein Names
Defensin, beta 3
Protein Family
Beta-defensin
UniProt Gene Name
Defb3??[Similar Products]
UniProt Synonym Gene Names
Bd3??[Similar Products]
UniProt Entry Name
DEFB3_MOUSE
UniProt Comments for Defb3
Function: Antimicrobial activity against Gram-negative bacteria E.coli and P.aeruginosa. Ref.1
Subcellular location: Secreted.
Tissue specificity: Highest expression in salivary glands, epididymis, ovary and pancreas and to a lesser extent in lung, liver and brain. Low or no expression in skeletal muscle and tongue. Ref.1 Ref.3
Induction: By bacterial infection. Ref.1
Sequence similarities: Belongs to the beta-defensin family. LAP/TAP subfamily.
Research Articles on Defb3
1. Mouse beta-defensin 3 was strongly up-regulated in colonic epithelium of 15 (but not 5) week-old IL-2(-/-) mice and in dextran-sulfate-induced chronic (but not acute) colitis, but not in IL-2(+/-) 15-week-old mice.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
