Human ADAMTS-like protein 1 (ADAMTSL1) ELISA kit; RP11-220B22.2; ADAMTSR1; C9orf94; DKFZp686L03130; FLJ35283; FLJ41032; FLJ46891; MGC118803; MGC118805; MGC40193; PUNCTIN; ADAM-TS related protein 1; ADAMTS-like 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human ADAMTSL1. No significant cross-reactivity or interference between Human ADAMTSL1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADAMTSL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS912907 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAMTS-like 1 (ADAMTSL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTSL1. The ELISA analytical biochemical technique of the MBS912907 kit is based on ADAMTSL1 antibody-ADAMTSL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTSL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTSL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

193,409 Da

ADAMTS-like 1

ADAMTS-like protein 1; punctin-1; ADAM-TS related protein 1

ADAMTS-like protein 1

ADAMTSR1; C9orf94; UNQ528/PRO1071; ADAMTSL-1??[Similar Products]

ATL1_HUMAN

This gene encodes a secreted protein and member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

ADAMTSL1: a secreted protein and member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) family. This protein lacks the metalloproteinase and disintegrin-like domains, which are typical of the ADAMTS family, but contains other ADAMTS domains, including the thrombospondin type 1 motif. This protein may have important functions in the extracellular matrix. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: 9p21.3

Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen

Molecular Function: metallopeptidase activity; zinc ion binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; post-translational protein modification; proteolysis

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