Human N-acetylserotonin O-methyltransferase-like protein (ASMTL) ELISA kit; RP11-261P4.3; ASMTLX; ASMTLY; ASTML; acetylserotonin N-methyltransferase-like; acetylserotonin O-methyltransferase-like

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between Human ASMTL and analogues was observed.

Store all reagents at 2-8 degree C

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Small volumes of ASMTL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9328831 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the acetylserotonin O-methyltransferase-like (ASMTL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASMTL. The ELISA analytical biochemical technique of the MBS9328831 kit is based on ASMTL antibody-ASMTL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASMTL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASMTL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ASMTL concentrations in Human serum, plasma and other body fluids. Using Purified Human ASMTL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ASMTL and ASMTL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ASMTL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

68,857 Da

acetylserotonin O-methyltransferase-like

N-acetylserotonin O-methyltransferase-like protein; acetylserotonin N-methyltransferase-like

N-acetylserotonin O-methyltransferase-like protein

ASMTL??[Similar Products]

ASML_HUMAN

The protein encoded by this gene has an N-terminus that is similar to the multicopy associated filamentation (maf) protein of Bacillus subtilis and to orfE of Escherichia coli, while the C-terminus is similar to N-acetylserotonin O-methyltransferase. This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

ASMTL: Unknown. The presence of the putative catalytic domain of S-adenosyl-L-methionine binding argues for a methyltransferase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: Xp22.3; Yp11.3

Cellular Component: cytoplasm

Molecular Function: O-methyltransferase activity

Biological Process: methylation

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