Inhibin beta A chain; inhibin, beta A; Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain

For Research Use Only. Not for use in diagnostic procedures.

Natural and recombinant human Activin A

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

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Small volumes of INHBA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS175781 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activin A (INHBA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INHBA. The ELISA analytical biochemical technique of the MBS175781 kit is based on INHBA antibody-INHBA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHBA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHBA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: The Human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(Expression system for standard: CHO ; Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. Acvidin-Biotin-Peroxidase Compiex was added and unbound conugates were washed away with PBS or TBS buffer HRP substrate TMB was used to visualize HRP enzymatic tion. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Activin A amount of sample captured in plate.

Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of Ire transforming growth factor-beta superfamily, is expressed loca!ly by the mesenchymal component of the hemopoietlc microenvironment. its expression is regulated on the mRNA level by different cytoKines. and the biologilt activity of the protein is... controlled by several inhibitory molecules.' Inhibins and activins are members of ine transforming growth factor beta superfamily and are known to modulate the growth and differentiation of severe I cell types 2 Inhibins and activins inhibit and activate. respectively. the secretion of follitropin by the pituitary glanj. Inhibins/activins are Involved in regulating a number of diverse functions such as hypothalamic and pituitary horlnone secretion, gonadal hormone secretion, germ celi development and maturation. erythroid differentiation. insulin secretion. nerve cell survival embryonic axial development or bone growth depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Human Activin A PicoKine ELISA Kit standard curve

47,442 Da

inhibin, beta A

inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)

Inhibin, beta A (Activin A, activin AB alpha polypeptide)

A4D1W7_HUMAN

The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]

INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; hair follicle development; cell-cell signaling; ovarian follicle development; erythrocyte differentiation; cell differentiation; cell cycle arrest; negative regulation of B cell differentiation; hemoglobin biosynthetic process; response to drug; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; growth; G1/S transition of mitotic cell cycle

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