Anti -Lipoprotein Receptor-related Protein 6, Low Density (LRP6, FLJ90062, FLJ90421)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (169983619..170219123, complement). Location: 2q31.1

Monoclonal

IgG

10E36

Rabbit

Recongnizes endogenous levels of human total LRP6 protein. Species crossreactivity

Purified
Purified

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-LRP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density-lipoprotein re-eptor (LDLR) related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/beta-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490 and Thr1493 by kinases such as GSK-3 and CK1 (8-10). The phosphorylated LRP6 recruits axin to the membrane, and presumably activates beta-catenin signaling (8-10).

Antibodies; Abs to Membrane Proteins

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:200

521,958 Da[Similar Products]

low density lipoprotein receptor-related protein 2

low-density lipoprotein receptor-related protein 2; LRP-2; megalin; glycoprotein 330; calcium sensor protein; Heymann nephritis antigen homolog

Low-density lipoprotein receptor-related protein 2

LRP-2; gp330??[Similar Products]

LRP2_HUMAN

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi apparatus; brush border membrane; endoplasmic reticulum; endocytic vesicle; lysosome; lysosomal membrane; apical plasma membrane; plasma membrane; integral to membrane; coated pit; endosome; receptor complex

Molecular Function: protein binding; calcium ion binding; SH3 domain binding

Biological Process: steroid metabolic process; cell proliferation; receptor-mediated endocytosis; phototransduction, visible light; forebrain development; protein amino acid glycosylation; endocytosis; lipid metabolic process; retinoid metabolic process; vitamin D metabolic process

Disease: Donnai-barrow Syndrome

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