Anti -Lipoprotein Receptor-related Protein 5, Low Density (LRP5)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 2; NC_000002.11 (169983619..170219123, complement). Location: 2q31.1

Polyclonal

IgG

Rabbit

Recognizes human LRP5 protein. Western Blot tests using negative control mock pSEC-tag-transfected 293T cell lysates did not yield the target band. Species Crossreactivity: Mouse.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-LRP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

WNT proteins have diverse roles in governing cell fate, proliferation, migration, porarity and death. WNT signaling controls development and is important for embryogenesis and carcinogenesis (1-3). WNTs are transduced through at least three distinct intracellular signaling pathways including the canonical 'WNT/b-catenin', 'WNT/Ca2+' and 'WNT' polarity (also called the 'planar polarity' pathway) (2,4,5) pathways. LRP5 (Low Density Lipoprotein Receptor-related Protein 5) is a member of the LDL receptor family, binds apolipoprotein E, and is widely expressed in many tissues, including hepatocytes, adrenal gland, and pancreas (6). LRP5 functions as a WNT co-receptor and interacts with Frizzled receptors. LRP5 is important in embryonic development, bone accrual development, eye development, normal cholesterol metabolism, and glucose-induced insulin secretion (6). Expression of LRP5 has also been used as a novel marker for disease progression in high-grade osteosarcoma (7).

Antibodies; Abs to Receptors

Western Blot (WB), Immunofluorescence (IF)

Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 2-3ug/ml; Note: For optimal results in immunofluorescence assays, cells should be fixed in 4% paraformaldehyde/0.5% Triton X-100/PBS for 10 minutes, permeabilized with 0.5% Triton X-100/PBS for 5 minutes, and blocked with 5% BSA/PBS for 30 minutes.
Western Blot: 1-3ug/ml; Single band at ~200kD

521,958 Da[Similar Products]

low density lipoprotein receptor-related protein 2

low-density lipoprotein receptor-related protein 2; LRP-2; megalin; glycoprotein 330; calcium sensor protein; Heymann nephritis antigen homolog

Low-density lipoprotein receptor-related protein 2

LRP-2; gp330??[Similar Products]

LRP2_HUMAN

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi apparatus; lysosome; brush border membrane; lysosomal membrane; endocytic vesicle; endoplasmic reticulum; apical plasma membrane; plasma membrane; integral to membrane; coated pit; receptor complex; endosome

Molecular Function: protein binding; calcium ion binding; SH3 domain binding

Biological Process: steroid metabolic process; receptor-mediated endocytosis; phototransduction, visible light; cell proliferation; forebrain development; protein amino acid glycosylation; endocytosis; lipid metabolic process; retinoid metabolic process; vitamin D metabolic process

Disease: Donnai-barrow Syndrome

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